Standard

Rev. 10/2002

Rev. 02/2004

VET 102 Diagnostic Laboratory Procedures I

Course Description

Presents an introduction to the principles and procedures for the veterinary practice laboratory. Emphasis is placed on laboratory safety; handling specimens; technical skills in hematology, cytology, clinical chemistry, serology, parasitology, and radiology; maintaining laboratory equipment; and quality control principles and practices. Topics include: handling of laboratory specimens and laboratory safety, principles of hematology and cytology, clinical chemistry, principles of serology, principles of urinalysis, principles of parasitology, and principles of radiology.

 

Competency Areas

Hours

 

Handling of Laboratory Specimens and

Class

2

 Laboratory Safety

D. Lab

6

Principles of Hematology and Cytology

P. Lab/O.B.I.

0

Clinical Chemistry

Credit

5

Principles of Serology

 

 

Principles of Urinalysis

 

 

Principles of Parasitology

 

 

Principles of Radiology

 

 

 

Prerequisite/ Corequisite:

CHM 191, VET 101

 

Course Guide

 

Competency

After completing this section, the student will:

Hours

Class

D.Lab

P.Lab/

O.B.I.

HANDLING OF LABORATORY SPECIMENS AND LABORATORY SAFETY

4

2

0

Safety

Explain the Hazardous Chemical Right to Know law.

 

 

 

 

Explain basic principles of laboratory safety including rabies suspect and sample handling.

 

 

 

Specimens

Prepare anticoagulated blood for hematology.

 

 

 

 

Harvest serum from clotted blood.

 

 

 

 

Package specimens for shipment.

 

 

 

 

Harvest biologic specimens for parasite exam.

 

 

 

PRINCIPLES OF HEMATOLOGY AND CYTOLOGY        

2

13

0

Stains and smears

Prepare and stain blood films, and exudate smears and sediment smears of bodily fluids.

 

 

 

 

Prepare routine blood and microbiologic stains.

 

 

 

Cell counters

Use automated cell counters that include the capacity to determine cell counts, hemoglobin concentration, hematocrit, and red cell indices.

 

 

 

 

Calculate RBC indices.

 

 

 

 

Discuss quality control procedures for operating and properly maintaining automated cell counters.

 

 

 

Cell and platelet counts

Perform red and white cell counts and platelet count on blood and bodily fluids using manual and automated methods.

 

 

 

Blood and other bodily fluid tests

Perform microhematocrit by centrifugal method on blood and other bodily fluids.

 

 

 

 

Perform plasma protein by refractometry on blood and other bodily fluids.

 

 

 

 

Perform viscosity and mucin precipitate tests on synovial fluid.

 

 

 

 

Determine color and clarity of bodily fluids before and after centrifugation.

 

 

 

 

Harvest citrated plasma for coagulation studies and prepare for shipping.

 

 

 

CLINICAL CHEMISTRY

6

3

0

Reference values, standards, and controls

Discuss derivation and use of reference values, standards, and controls.

 

 

 

Tests

Perform routine blood chemistry tests using a dry chemistry instrument.

 

 

 

Quality control procedures

Prepare and interpret a Levey-Jennings plot quality control procedure.

 

 

 

 

Describe the maintenance requirements and quality control procedures for monitoring centrifuges, incubator blocks or baths, and chemistry instruments.

 

 

 

PRINCIPLES OF SEROLOGY

0

2

0

Serology

Perform serologic tests using the ELISA method.

Perform a slide or card agglutination test.

 

 

 

PRINCIPLES OF URINALYSIS

0

3

0

Urinalysis

Perform urinalysis including determination of color, clarity, and specific gravity.

 

 

 

 

Perform dipstick and tablet urine chemistry tests.

 

 

 

 

Set up sample for urine sediment examination.

 

 

 

PRINCIPLES OF PARASITOLOGY

0

3

0

Parasitology

Prepare solutions and perform fecal flotation procedure.

 

 

 

 

Prepare solutions and perform fecal sedimentation procedure.

 

 

 

PRINCIPLES OF RADIOLOGY

8

34

0

Safety

Discuss radiation safety measures.

 

 

 

Maintenance and operational procedures

Label, file, and store film.

 

 

 

 

Store and handle gloves and aprons.

 

 

 

 

Replace or replenish developer and fixer.

 

 

 

 

Clean radiograph screens.

 

 

 

Processing

Process radiographs in darkroom using manual and automated methods.

 

 

 

 

Use technique charts and stationary and portable x-ray machines.

 

 

 

 

Position animal patients for taking routine diagnostic radiographs.

 

 

 

 

Suggested Resources

 

Books

Bowman, D.  (2003). Georgisparasitology for veterinarians.  (8th ed.)  Philadelphia: Saunders.

Carter, G. & Cole, J. (1990).  Diagnostic procedures in veterinary bacteriology and mycology.  (5th ed.)  New York: Academic Press.

Chew, D. & DiBartola, S.  (1998).  Interpretation of canine and feline urinalysis.  Wilmington, DE: The Gloyd Group.

Crow, S. & Walshaw, S.  (1997).  Manual of clinical procedures in the dog, cat & rabbit.  (2nd ed.)  Philadelphia:  Lippincott, Williams & Wilkins.

Dorland.  (2004).  Dorland’s pocket dictionary.  (27th ed.)  Philadelphia: Saunders.

Foreyt, W. (2001).  Veterinary parasitology.  (5th  ed.)  Ames, IA: Blackwell.

Han, C. & Hurd, C.  (2005).  Practical diagnostic imaging for the veterinary technician.  (3rd ed.)  St. Louis: Mosby.

Harvey, J. (2001).  Atlas of veterinary hematology: Blood and bone marrow of domestic animals.  Philadelphia: Saunders.

Hendrix, C. (2002).  Laboratory procedures for veterinary technicians.  (4th ed.)  St. Louis: Mosby.

Ikram, M. (1994).  Microbiology for veterinary technicians.  St. Louis: Mosby.

Lavin, L. (2003).  Radiography in veterinary technology.  (3rd ed.)  Philadelphia: Saunders.

McCurnin, D. & Bassert, J. (2002).  Clinical textbook for veterinary technicians.  (5th ed.)  Philadelphia: Saunders.

Reagan, W., Sanders, T. & DeNicola, D.  (1998).  Veterinary hematology: Atlas of common domestic species.  Ames, IA: Blackwell.

Rebar, A.  (1998).  Hemogram interpretation for dogs and cats.  Wilmington, DE: The Gloyd Group.

Rebar, A., MacWilliams, P., Feldman, B., Metzgar, F. & Pollack, R.  (2001).  A guide to hematology in dogs and cats.  Jackson, WY: Teton NewMedia.

Sirois, M.  (1995).  Veterinary clinical laboratory procedures.  St. Louis: Mosby.

Thrall, M.  (2004).  Veterinary hematology and clinical chemistry.   Philadelphia: Lippincott, Williams & Wilkins.