Standard |
BIO 197 - Introductory
Microbiology
Provides students with a foundation in basic microbiology with emphasis on infectious diseases. Topics include: characterization, classification, and description of microorganisms; use of compound microscope; morphology and fine structure of bacteria; gram positive and gram negative bacteria; reproduction and growth of bacteria; viral diseases; host-parasite relationship; host defense mechanisms; epidemiology; antimicrobial and chemotherapeutic agents; control of microorganisms; and laboratory safety.
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Competency
Areas |
Hours |
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Characterization, Classification, and Description of Microorganisms |
Class |
3 |
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Use of Compound Microscope |
D. Lab |
4 |
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Morphology and Fine Structure of Bacteria |
P. Lab/O.B.I. |
0 |
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Gram Positive and Gram Negative Bacteria |
Credit |
5 |
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Reproduction and Growth of Bacteria |
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Viral Diseases |
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Host-Parasite Relationship |
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Host Defense Mechanisms |
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Epidemiology |
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Antimicrobial and Chemotherapeutic Agents |
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Control of Microorganisms |
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Laboratory Safety |
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Prerequisite/Corequisite: |
BIO 193 |
Course Guide |
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Competency |
After completing this
section, the student will: |
Hours | ||
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Class |
D.Lab |
P.Lab/
O.B.I. | ||
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CHARACTERIZATION,
CLASSIFICATION, AND DESCRIPTION OF MICROORGANISMS |
5 |
11 |
0 | |
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Characterization |
Explain characterization of organisms. |
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Classification |
Classify organisms. |
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Staining techniques |
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Use staining techniques to classify specific organisms. |
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Identification |
Identify common microorganisms from their chemical and physical properties. |
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USE OF COMPOUND MICROSCOPE |
1 |
5 |
0 | |
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Operation |
Operate a compound light microscope. |
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Maintenance |
Maintain a compound light microscope. |
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MORPHOLOGY AND FINE STRUCTURE OF BACTERIA |
2 |
0 |
0 | |
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Summarize the morphology of bacteria. |
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Fine structure |
Explain the fine structure of bacteria. |
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GRAM POSITIVE AND GRAM NEGATIVE
BACTERIA |
3 |
5 |
0 | |
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Bacteria |
Identify common gram negative and gram positive bacteria. |
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Properties and morphology |
Describe bacteria with unusual properties and complex morphology. |
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REPRODUCTION AND GROWTH OF
BACTERIA |
3 |
5 |
0 | |
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Reproduction |
Explain the reproduction of bacteria. |
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Growth |
Describe bacteria growth. |
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Cultivation |
Cultivate specific microorganisms in various media. |
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Determine the exact number of microorganisms in a culture medium by both direct and indirect methods. |
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VIRAL DISEASES |
3 |
0 |
0 | |
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Replication |
Explain the replication of viruses. |
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Structure and function |
Describe the structure and function of viruses. |
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Pathogenic viruses |
Discuss significant viral pathogens. |
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Respiratory system |
Describe the characteristics of viruses that affect the human respiratory system. |
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Describe factors impacting respiratory system viral disease development. |
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HOST-PARASITE RELATIONSHIP |
2 |
0 |
0 | |
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Infection |
Describe factors that affect the ability of the host to resist infection. |
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Describe host innate or natural defense mechanisms. |
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Discuss the effects of immunosuppressive drug therapy, concurrent disease, and aging on the host-parasite relationship. |
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Antibodies |
Discuss the role of antibodies and cellular immunity. |
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Mode of entry |
Explain ways in or conditions under which a pathogenic agent can enter the body. |
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Evasion |
Describe the evasion mechanisms of the pathogen. |
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HOST DEFENSE MECHANISMS |
2 |
0 |
0 | |
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Host response |
Discuss nonspecific host defenses that offer resistance to microorganism establishment. |
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Discuss specific host defenses that offer resistance to microorganism establishment, including antibody formation, active and passive immunity, and natural and acquired immunity. |
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Discuss the disorders of the immune system. |
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Differentiate between innate and acquired immunity. |
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EPIDEMIOLOGY |
2 |
0 |
0 | |
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Epidemiology |
Discuss incidence versus prevalence. |
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Describe serum epidemiology. |
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Describe indirect and direct disease transmission methods. |
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ANTIMICROBIAL AND CHEMOTHERAPEUTIC AGENTS |
3 |
4 |
0 | |
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Antibiotics and chemotherapeutic agents |
Discuss the methods by which various antibiotics and chemotherapeutic agents affect microorganisms. |
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Isolate organisms resistant to antibiotics using the gradient plate technique. |
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Cultivation and identification |
Determine antibiotic sensitivity of selected organisms using the Kirby-Bauer technique. |
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Isolate antibiotic-producing Streptomyces from soil. |
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CONTROL OF MICROORGANISMS |
3 |
5 |
0 | |
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Physical factors used in controlling microorganisms |
Determine the phenol coefficient of various disinfectants. |
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Study the death rate of bacterial cultures exposed to UV light. |
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Chemical methods to control microorganisms |
Determine the impact of various disinfectants and antiseptics on microorganisms. |
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Disinfection and sterilization in laboratory situations |
Discuss practical applications of various disinfection and sterilization procedures. |
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Evaluate sterility testing procedures. |
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LABORATORY SAFETY |
1 |
5 |
0 | |
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Safety procedures |
Recognize safety procedures in a microbiological laboratory. |
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Adhere to safety procedures in a microbiological laboratory. |
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Record data in a scientific notebook. |
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Prepare a laboratory report. |
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Suggested Resources |
Atlas, R. M., & Brown, A. E. (1988). Experimental microbiology (2nd ed.). New
York: Macmillan.
Barnes, R. S. (Ed.). (1984). A synoptic classification of living
organisms. London: Blackwell Scientific.
Barrett, J. T. (Ed.). (1986). Contemporary classics in the life sciences:
Cell biology (Vol. 1). Philadelphia: ISI Press.
Barrett, J. T. (Ed.). (1986). Contemporary classics in the life sciences:
The molecules of life (Vol. 2). Philadelphia: ISI
Press.
Buchanan, R. E., & Gibbons, N. F. (Eds.).
(1974). Bergey's manual of determinative
bacteriology (8th ed.). Baltimore: Williams &
Wilkins.
Cano, R. J., & Colomé, J. S. (1986). Microbiology. St. Paul:
West.
Cappuccino, J. G., & Sherman, N. (1987).
Microbiology: A laboratory manual
(2nd ed.). Reading, MA: Addison-Wesley.
DIFCO Laboratories Staff. (1984). DIFCO manual: Dehydrated culture media and
reagents for microbiology (10th ed.). Detroit: Author.
Fawcett, D. W. (1981). The cell (2nd ed.). Philadelphia:
Saunders.
Gilstrap, M., et al. (1982). Microbiology experiments: A health science
perspective. Philadelphia: Saunders College.
Hewitt, W., & Vincent, S. (1988). Theory and application of microbiological
assay. San Diego: Academic Press.
Jensen, J. B. (Ed.). (1983). In vitro cultivation of protozoan
parasites. Boca Raton, FL: CRC Press.
Jones, R. M. (1966). Basic microscopic technics. Chicago:
University of Chicago Press.
Kavanagh, F. (1963). Analytical microbiology. New York:
Academic Press.
Kerr, T. J. (1990). Applications in general microbiology
(4th ed.). Salem, NC: Hunter Texts.
Kirsop, B. E., & Snell, J. J. (Eds.).
(1984). Maintenance of microorganisms: A
manual of laboratory methods. Orlando, FL: Academic
Press.
Krieg, N. R., & Holt, J. G. (Eds.).
(1984). Bergey's manual of systematic
bacteriology (Vol. 1). Baltimore: Williams &
Wilkins.
Laskin, A. I., & Lechevalier, H. A.
(Eds.). (1977). CRC handbook of
microbiology bacteria (Vol. 1) (2nd ed.). Boca Raton, FL: CRC
Press.
Miller, B. M. (Ed.). (1986). Laboratory safety: Principles and
practices. Washington, DC: American Society for
Microbiology.
Nurse, P., & Streiblová, E. (Eds.).
(1984). The microbial cell cycle.
Boca Raton, FL: CRC Press.
O'Leary, W. (Ed.). (1989). Practical handbook of microbiology. Boca
Raton, FL: CRC Press.
Pelczar, M. J., et al. (1986). Microbiology (5th ed.). New York:
McGraw-Hill.
Primrose, S. B., & Wardlaw, A. C. (1982).
Sourcebook of experiments for the
teaching of microbiology. Orlando, FL: Academic Press.
Scaife, J., et al. (1985). Genetics of bacteria. Orlando, FL:
Academic Press.
Sneath, P. H., et al. (Eds.). (1986). Bergey's manual of systematic
bacteriology (Vol. 2). Baltimore: Williams &
Wilkins.
Windholz, M., et al. (Eds.). (1983). The Merck index (10th ed.). Rahway, NJ: Merck.